Bcl-2 Polyclonal Antibody（E-AB-60012）|-Elabscience伊莱瑞特【官网】

Bcl-2 Polyclonal Antibody

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货号：E-AB-60012
文献（51）
说明书

说明书

Price: ¥3580.00 ¥2220.00 ¥1380.00

Size:

200 μL 120 μL 60 μL

宿主： Rabbit
反应性： Human;Mouse;Rat
应用性： WB;IHC;IF

Verified Samples	Verified Samples in WB: HL-60, HL-60, Mouse testis, Rat lung, Rat liver Verified Samples in IHC: Human lung cancer, Rat brain, Mouse testis Verified Samples in IF: C6, NIH/3T3, U-2OS
Dilution	WB 1:500-1:2000, IHC 1:50-1:100, IF 1:50-1:100
Clonality	Polyclonal
Isotype	IgG
Immunogen	A synthetic peptide of human Bcl-2
Abbre	Bcl-2
Synonyms	BCL2, Bcl-2, PPP1R50
Swissprot	P10415
Calculated MW	22 kDa/26 kDa
Observed MW	24kDa/26 kDa
Cellular Localization	Mitochondrion outer membrane. Nucleus membrane. Endoplasmic reticulum membrane.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Purification Method	Affinity purification
Research Areas	Cancer, Cell Biology, Metabolism, Signal Transduction
Conjugation	Unconjugated
Background	This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.

Western blot analysis of extracts of HL-60 cells using Bcl-2 Polyclonal Antibody at dilution of 1:500.

Western blot analysis of extracts of various cells using Bcl-2 Polyclonal Antibody at dilution of 1:1000.

Immunohistochemistry of paraffin-embedded Human lung cancer using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded Rat brain using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded Mouse testis using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Chemopreventive Effects of Onosma mutabilis against Azoxymethane-Induced Colon Cancer in Rats via Amendment of Bax/Bcl-2 and NF-κB Signaling Pathways

IF: 3.100

Journal： CURRENT ISSUES IN MOLECULAR BIOLOGY (2023)

DOI：10.3390/cimb45020057

PMID：36826002

Reactivity：Rat

Sample Type：colonic tissue
Hyperbilirubinemia Aggravates Renal Ischemia Reperfusion Injury by Exacerbating PINK1- PARKIN-mediated Mitophagy

IF: 3.1

Journal： SHOCK (2023)

DOI：10.1097/SHK.0000000000002160

PMID：37278995

Reactivity：Mouse

Sample Type：renal cortical tissue
Morphological and molecular changes in the Harderian gland of streptozotocin-induced diabetic rats

IF: 2.8

Journal： Journal of Experimental Zoology Part A-Ecological and Integrative Physiology (2023)

DOI：10.1002/jez.2741

PMID：37522474

Reactivity：Rat

Sample Type：Harderian gland (HG)
SIRT1/Nrf2/NF-κB Signaling Mediates Anti-Inflammatory and Anti-Apoptotic Activities of Oleanolic Acid in a Mouse Model of Acute Hepatorenal Damage

IF: 2.6

Journal： Medicina-Lithuania (2023)

DOI：10.3390/medicina59071351

PMID：37512162

Reactivity：Mouse

Sample Type：liver tissue,kidney tissue
Pinostrobin attenuates azoxymethane-induced colorectal cytotoxicity in rats through augmentation of apoptotic Bax/Bcl-2 proteins and antioxidants

IF: 2.3

Journal： SAGE Open Medicine (2023)

DOI：10.1177/20503121231216585

PMID：38078205

Reactivity：Rat

Sample Type：colon tissue
Alleviation of adipose-hepatic glycolipid dysregulation by acetate in experimental PCOS model is associated with NF-κB/NLRP3 repression

IF: 2.1

Journal： CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2023)

DOI：10.1139/cjpp-2023-0169

Reactivity：Rat

Sample Type：adipose tissue,hepatic tissue
SERCA2a directs the cardioprotective role of nano-emulsion curcumin against PM2.5-induced cardiac injury in rats by prohibiting PERK-eIF2α pathway

IF: 6.780

Journal： LIFE SCIENCES (2022)

DOI：10.1016/j.lfs.2022.121160

Reactivity：Rat

Sample Type：cardiac tissues
First Evidence of the Expression and Localization of Prothymosin α in Human Testis and Its Involvement in Testicular Cancers

IF: 6.064

Journal： Biomolecules (2022)

DOI：10.3390/biom12091210

PMID：36139050

Reactivity：Human

Sample Type：testicular tissue
Semaphorin 3A attenuates the hypoxia suppression of osteogenesis in periodontal ligament stem cells

IF: 3.946

Journal： JOURNAL OF PERIODONTAL RESEARCH (2022)

DOI：10.1111/jre.12973

PMID：35037251

Reactivity：Human
Humanin Ameliorates Late-onset Hypogonadism in Aged Male Rats

IF: 3.855

Journal： Current Molecular Pharmacology (2022)

DOI：10.2174/1874467215666220127115602

Reactivity：Rat

Sample Type：testicular tissue

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