BRCA2 Polyclonal Antibody-Elabscience伊莱瑞特【官网】

BRCA2 Polyclonal Antibody

说明书

Price: ¥2100.00 ¥800.00

Size:

100 μL 25 μL

Verified Samples	Verified Samples in WB: Hela, Rat testis, Jurkat, MCF-7, A431 Verified Samples in IHC: Human colon, Human breast Verified Samples in IF: U-2OS, U-251MG
Dilution	WB 1:1000-1:3000, IHC 1:100-1:200, IF 1:100-1:400
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant Human Breast cancer type 2 susceptibility protein
Abbre	BRCA2
Synonyms	BRCC 2, BROVCA2, Breast and ovarian cancer susceptibility gene early onset antibody, FACD, FAD 1, FANCB, GLM3, BRCA 2
Swissprot	P51587
Calculated MW	384 kDa
Observed MW	384 kDa
Cellular Localization	Nucleus, Cytoskeleton.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4
Purification Method	Antigen Affinity Purification
Research Areas	Cancer, Epigenetics and Nuclear Signaling
Conjugation	Unconjugated
Background	Inherited mutations in BRCA1 and this gene, BRCA2, confer increased lifetime risk of developing breast or ovarian cancer. Both BRCA1 and BRCA2 are involved in maintenance of genome stability, specifically the homologous recombination pathway for double-strand DNA repair. The BRCA2 protein contains several copies of a 70 aa motif called the BRC motif, and these motifs mediate binding to the RAD51 recombinase which functions in DNA repair. BRCA2 is considered a tumor suppressor gene, as tumors with BRCA2 mutations generally exhibit loss of heterozygosity (LOH) of the wild-type allele.

Western Blot analysis of Jurkat, MCF-7 and A431 cells using BRCA2 Polyclonal Antibody at dilution of 1:2000

Western Blot analysis of Hela cells using BRCA2 Polyclonal Antibody at dilution of 1:2000

Western Blot analysis of Rat testis using BRCA2 Polyclonal Antibody at dilution of 1:2000

Immunohistochemistry of paraffin-embedded Human colon using BRCA2 Polyclonal Antibody at dilution of 1:100

Immunohistochemistry of paraffin-embedded Human breast using BRCA2 Polyclonal Antibody at dilution of 1:100

Immunofluorescence analysis of U-2OS cells using BRCA2 Polyclonal Antibody at dilution of 1:100

Immunofluorescence analysis of U-251MG cells using BRCA2 Polyclonal Antibody at dilution of 1:100

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E-AB-1010	Goat Anti-Rabbit IgG(H+L)(Cyanine3 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1014	Goat Anti-Rabbit IgG(H+L)(FITC conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1043	Streptavidin(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1060	Goat Anti-Rabbit IgG(H+L)(Elab Fluor® 594 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1075	Goat Anti-Rabbit IgG(H+L)(Elab Fluor® 647 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1099	Elab Fluor® Violet 450-Goat Anti-Rabbit IgG(H+L)	500 μL , 120 μL , 1 mL
E-AB-1194	HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed	500 μL , 120 μL , 1 mL
E-IR-R217	2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution)	50 mL , 18 mL , 6 mL , 3 mL
E-IR-R220	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0)	10mL , 3mL
E-IR-R221	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0)	10mL , 3mL
E-IR-R304A	Western Blot Detection Kit	50 Assays
E-IR-R304B	High Accuracy and Absorbability Western Blot Detection Kit	50 Assays