EGFR Polyclonal Antibody
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- 宿主： Rabbit
- 反应性： Human;Mouse;Rat
- 应用性： WB;IHC-p;IF
| Verified Samples |
Verified Samples in WB: 293 |
| Dilution | WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthesized peptide derived from human EGFR around the non-phosphorylation site of Thr693. |
| Abbre | EGFR |
| Synonyms | Avian erythroblastic leukemia viral (v erb b) oncogene homolog, Cell growth inhibiting protein 40, Cell proliferation inducing protein 61, EGF R, EGFR, Epide, Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) |
| Swissprot | |
| Calculated MW | 134 kDa |
| Observed MW | 180 ,134kDa |
| Cellular Localization | Secreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. Endosome membrane. Nucleus. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF). |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH 7.4. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Signal Transduction |
| Conjugation | Unconjugated |
| Background | Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.Isoform 2 may act as an antagonist of EGF action. |
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