EIF2 alpha Polyclonal Antibody-Elabscience伊莱瑞特【官网】

EIF2 alpha Polyclonal Antibody

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货号：E-AB-31295
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说明书

说明书

Price: ¥2365.00 ¥1640.00 ¥1010.00 ¥420.00

Size:

200 μL 120 μL 60 μL 20 μL

宿主： Rabbit
反应性： Human;Mouse;Rat;Monkey
应用性： WB;IHC-p;IF

Verified Samples	Verified Samples in WB: MCF-7, A549, K562, HEK293 Verified Samples in IHC: Human liver cancer Verified Samples in IF: Rat kidney
Dilution	WB 1:500-1:2000, IHC 1:100-1:300, IF 1:50-1:200
Clonality	Polyclonal
Isotype	IgG
Immunogen	Synthesized peptide derived from human eIF2α around the non-phosphorylation site of Ser51.
Abbre	eIF2α
Synonyms	EIF 2, EIF 2 alpha, EIF 2A, EIF 2alpha, EIF-2alpha, EIF2, EIF2 alpha, EIF2A, EIF2S1, Eukaryotic translation initiation fa, Eukaryotic translation initiation factor 2 subunit 1 alpha, Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa, eIF-2-alpha, eIF-2A
Swissprot	P05198
Calculated MW	36 kDa
Observed MW	38 kDa
Cellular Localization	Cytoplasmic granule. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity).
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH 7.4.
Purification Method	Affinity purification
Research Areas	Cancer, Epigenetics and Nuclear Signaling
Conjugation	Unconjugated
Background	Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins.

Western Blot analysis of various cells using EIF2 alpha Polyclonal Antibody at dilution of 1:1000.

Immunohistochemistry of paraffin-embedded Human liver cancer tissue using EIF2 alpha Polyclonal Antibody at dilution of 1:200.

Immunofluorescence analysis of Rat kidney tissue using EIF2 alpha Polyclonal Antibody at dilution of 1:200.

Eukaryotic Initiation Translation Factor 2A activation by cannabidiolic acid alters the protein homeostasis balance in glioblastoma cells

IF: 7.700

Journal： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024)

DOI：10.1016/j.ijbiomac.2024.132968

Reactivity：Human

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E-AB-1010	Goat Anti-Rabbit IgG(H+L)(Cyanine3 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1014	Goat Anti-Rabbit IgG(H+L)(FITC conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1043	Streptavidin(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1060	Goat Anti-Rabbit IgG(H+L)(Elab Fluor® 594 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1075	Goat Anti-Rabbit IgG(H+L)(Elab Fluor® 647 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1099	Elab Fluor® Violet 450-Goat Anti-Rabbit IgG(H+L)	500 μL , 120 μL , 1 mL
E-AB-1194	HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed	500 μL , 120 μL , 1 mL
E-IR-R217	2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution)	50 mL , 18 mL , 6 mL , 3 mL
E-IR-R220	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0)	10mL , 3mL
E-IR-R221	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0)	10mL , 3mL
E-IR-R304A	Western Blot Detection Kit	50 Assays
E-IR-R304B	High Accuracy and Absorbability Western Blot Detection Kit	50 Assays