ERK 1/2 Polyclonal Antibody（E-AB-31374）|-Elabscience伊莱瑞特【官网】

ERK 1/2 Polyclonal Antibody

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货号：E-AB-31374
文献（12）
说明书

说明书

Price: ¥2365.00 ¥1640.00 ¥1010.00 ¥420.00

Size:

200 μL 120 μL 60 μL 20 μL

宿主： Rabbit
反应性： Human;Mouse;Rat
应用性： WB;IHC-p;IF

Verified Samples	Verified Samples in WB: K562 Verified Samples in IHC: Human liver cancer Verified Samples in IF: Rat lung
Dilution	WB 1:500-1:2000, IHC 1:100-1:300, IF 1:50-1:200
Clonality	Polyclonal
Isotype	IgG
Immunogen	Synthesized peptide derived from the C-terminal region of human ERK 1/2
Abbre	ERK 1/2
Synonyms	ERK-1, ERK1, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 3, MAP kinase isoform p44, MAPK 3, MAPK3, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 3, PRKM3, p, p44-MAPK
Swissprot	P27361, P28482
Calculated MW	43 kDa
Observed MW	42 ,44kDa
Cellular Localization	Nucleus.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH 7.4.
Purification Method	Affinity purification
Research Areas	Cancer, Signal Transduction, Neuroscience
Conjugation	Unconjugated
Background	Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.

Western Blot analysis of K562 cells using ERK 1/2 Polyclonal Antibody at dilution of 1:2000.

Immunohistochemistry of paraffin-embedded Human liver cancer tissue using ERK 1/2 Polyclonal Antibody at dilution of 1:200.

Immunofluorescence analysis of Rat lung tissue using ERK 1/2 Polyclonal Antibody at dilution of 1:200.

PTX3 Predicts Myocardial Damage and Fibrosis in Duchenne Muscular Dystrophy

IF: 3.367

Journal： Frontiers in Physiology (2020)

DOI：10.3389/fphys.2020.00403

PMID：32508664

Reactivity：Mouse

Sample Type：Tissue homogenate
Fermented Unpolished Black Rice (Oryza sativa L.) Inhibits Melanogenesis via ERK, p38, and AKT Phosphorylation in B16F10 Melanoma Cells

IF: 1.992

Journal： JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY (2020)

DOI：10.4014/jmb.2003.03019

PMID：32423183

Reactivity：Mouse

Sample Type：Cell sample

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