FGF2 Polyclonal Antibody-Elabscience伊莱瑞特【官网】

FGF2 Polyclonal Antibody

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货号：E-AB-60031
文献（3）
说明书

说明书

Price: ¥3580.00 ¥2220.00 ¥1380.00

Size:

200 μL 120 μL 60 μL

宿主： Rabbit
反应性： Human;Mouse;Rat
应用性： WB;IHC;IF

Verified Samples	Verified Samples in WB: Rat ovary Verified Samples in IHC: Rat lung, Human prostate, Mouse heart Verified Samples in IF: C6, NIH/3T3
Dilution	WB 1:500-1:2000, IHC 1:50-1:200, IF 1:50-1:200
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant fusion protein of human FGF2 (NP_001997.5).
Abbre	FGF2
Synonyms	BFGF, Basic FGF, FGF-2, FGF2, FGFB, HBGF-2
Swissprot	P09038
Calculated MW	17 kDa/21 kDa/22 kDa/30 kDa
Observed MW	20 kDa
Cellular Localization	Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Purification Method	Affinity purification
Research Areas	Cancer, Cardiovascular, Developmental Biology, Neuroscience, Signal Transduction, Stem Cells
Conjugation	Unconjugated
Background	The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.

Western blot analysis of extracts of Rat ovary using FGF2 Polyclonal Antibody at dilution of 1:1000.

Immunohistochemistry of paraffin-embedded Rat lung using FGF2 Polyclonal Antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded Human prostate using FGF2 Polyclonal Antibody at dilution of 1:100 (20x lens).

Immunohistochemistry of paraffin-embedded Mouse heart using FGF2 Polyclonal Antibody at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using FGF2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using FGF2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Role of glycosphingolipid SSEA-3 and FGF2 in the stemness and lineage commitment of multilineage differentiating stress enduring (MUSE) cells

IF: 8.755

Journal： CELL PROLIFERATION (2022)

DOI：10.1111/cpr.13345

PMID：36225120

Reactivity：Human
Spinal Canal and Spinal Cord in Rat Continue to Grow Even after Sexual Maturation: Anatomical Study and Molecular Proposition

IF: 6.208

Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)

DOI：10.3390/ijms232416076

Reactivity：Rat

Sample Type：cervical spinal cords
Investigation of the potential therapeutic effect of cationic lipoplex mediated fibroblast growth factor-2 encoding plasmid DNA delivery on wound healing

IF: 3.117

Journal： DARU-Journal of Pharmaceutical Sciences (2021)

DOI：10.1007/s40199-021-00410-y

Reactivity：Mouse

E-AB-1003	Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1010	Goat Anti-Rabbit IgG(H+L)(Cyanine3 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1014	Goat Anti-Rabbit IgG(H+L)(FITC conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1043	Streptavidin(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1060	Goat Anti-Rabbit IgG(H+L)(Elab Fluor® 594 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1075	Goat Anti-Rabbit IgG(H+L)(Elab Fluor® 647 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1099	Elab Fluor® Violet 450-Goat Anti-Rabbit IgG(H+L)	500 μL , 120 μL , 1 mL
E-AB-1194	HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed	500 μL , 120 μL , 1 mL
E-IR-R217	2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution)	50 mL , 18 mL , 6 mL , 3 mL
E-IR-R220	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0)	10mL , 3mL
E-IR-R221	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0)	10mL , 3mL
E-IR-R304A	Western Blot Detection Kit	50 Assays
E-IR-R304B	High Accuracy and Absorbability Western Blot Detection Kit	50 Assays