Flag-Tag Polyclonal Antibody-Elabscience伊莱瑞特【官网】

Flag-Tag Polyclonal Antibody

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货号：E-AB-40525
文献（3）
说明书

说明书

Price: ¥780.00 ¥300.00

Size:

100 μL 25 μL

宿主： Rabbit
反应性： All
应用性： WB;IF;IP

Verified Samples	Verified Samples in WB: Flag-Tagged fusion protein Verified Samples in IP: Hela, 293F
Dilution	WB 1:10000-1:20000, IP 3ug/sample, IF 1:2000-4000
Clonality	Polyclonal
Isotype	IgG
Immunogen	Synthetic peptide corresponding to Flag tag conjugated to keyhole limpet haemocyanin.
Abbre	Flag-Tag
Synonyms	DDDDK epitope tag
Calculated MW	28 kDa
Observed MW	28 kDa
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4
Purification Method	Antigen Affinity Purification
Conjugation	Unconjugated
Background	FLAG-tag is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography.

Western blot analysis of 293F cells transfected with Flag tagged recombinant protein at different dilutions. lane 1: 1: 5000, lane 2: 1: 10000, lane 3: 1: 20000, lane 4: 1: 40000, lane 5: 1: 80000, lane 6: 1: 160000, lane 7: 1: 320000, lane 8: 1: 640000

Immunofluorescent analysis of 293F cells transfected with the FLAG-GFP, using anti-Flag-Tag Polyclonal Antibody at dilution of 1:12000.

IP Result of 293F cells transfected with FLAG-Tag fusion protein, using anti-FLAG-Tag rabbit antibody. Lane 1:input, lane 2: rabbit IgG Isotype Control, Lane 3: anti-FLAG-Tag rabbit antibody

Excitatory and inhibitory synapses show a tight subcellular correlation that weakens over development

IF: 7.500

Journal： Cell Reports (2024)

DOI：10.1016/j.celrep.2024.114361

Reactivity：Rat

Sample Type：hippocampal tissue
Roles of transcriptional factor PsrA in the regulation of quorum sensing in Pseudomonas aeruginosa PAO1

IF: 4.000

Journal： Frontiers in Microbiology (2024)

DOI：10.3389/fmicb.2024.1424330

Reactivity：Pseudomonas aeruginosa PAO1
Pattern recognition receptor mediated innate immune response requires a Rif-dependent pathway

IF: 14.511

Journal： JOURNAL OF AUTOIMMUNITY (2022)

DOI：10.1016/j.jaut.2022.102975

PMID：36527784

Reactivity：Human

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