GSK3 beta Polyclonal Antibody-Elabscience伊莱瑞特【官网】

GSK3 beta Polyclonal Antibody

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货号：E-AB-31629
文献（4）
说明书

说明书

Price: ¥2365.00 ¥1640.00 ¥1010.00 ¥420.00

Size:

200 μL 120 μL 60 μL 20 μL

宿主： Rabbit
反应性： Human;Mouse;Rat
应用性： WB;IHC-p;IF

Verified Samples	Verified Samples in WB: HT29 Verified Samples in IHC: Human liver cancer, Verified Samples in IF: Rat kidney
Dilution	WB 1:500-1:2000, IHC 1:100-1:300, IF 1:100-1:300
Clonality	Polyclonal
Isotype	IgG
Immunogen	Synthesized peptide derived from human GSK3β around the non-phosphorylation site of Ser9.
Abbre	GSK3β
Synonyms	GSK 3 beta, GSK-3 beta, GSK3B, GSK3beta isoform, Glycogen Synthase Kinase 3 Beta, Glycogen synthase kinase-3 beta, Serine/threonine-protein kinase GSK3B
Swissprot	P49841
Calculated MW	47 kDa
Observed MW	47 kDa
Cellular Localization	Cytoplasm. Nucleus. Cell membrane. The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH 7.4.
Purification Method	Affinity purification
Research Areas	Cancer, Cardiovascular, Metabolism, Neuroscience, Signal Transduction, Stem Cells
Conjugation	Unconjugated
Background	Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair.

Western Blot analysis of HT-29 cells using GSK3 beta Polyclonal Antibody at dilution of 1:2000.

Immunohistochemistry of paraffin-embedded Human liver cancer tissue using GSK3 beta Polyclonal Antibody at dilution of 1:200.

Immunofluorescence analysis of Rat kidney tissue using GSK3 beta Polyclonal Antibody at dilution of 1:200.

Phosphocreatine ameliorates hepatocellular apoptosis mediated by protecting mitochondrial damage in liver ischemia/reperfusion injury through inhibiting TLR4 and Agonizing Akt Pathway

IF: 2.700

Journal： TISSUE & CELL (2024)

DOI：10.1016/j.tice.2024.102599

Reactivity：Mouse,Human

Sample Type：liver tissue
Tea seed saponin?reduced extract ameliorates palmitic acid?induced insulin resistance in HepG2 cells

IF: 3.400

Journal： Molecular Medicine Reports (2023)

DOI：10.3892/mmr.2023.13149

PMID：38099345

Reactivity：Human
Royal jelly regulates the caspase, Bax and COX-2, TNF-α protein pathways in the fluoride exposed lung damage in rats

IF: 2.586

Journal： TISSUE & CELL (2022)

DOI：10.1016/j.tice.2022.101754

Reactivity：Rat

Sample Type：lung tissue
Sonodynamic Therapy Inhibits Fibrogenesis in Rat Cardiac Fibroblasts Induced by TGF-β1

IF: 4.652

Journal： CELLULAR PHYSIOLOGY AND BIOCHEMISTRY (2016)

DOI：10.1159/000452571

PMID：27889772

Reactivity：Rat

Sample Type：Cell culture supernatant

E-AB-1003	Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1010	Goat Anti-Rabbit IgG(H+L)(Cyanine3 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1014	Goat Anti-Rabbit IgG(H+L)(FITC conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1043	Streptavidin(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1060	Goat Anti-Rabbit IgG(H+L)(Elab Fluor^® 594 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1075	Goat Anti-Rabbit IgG(H+L)(Elab Fluor^® 647 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1099	Elab Fluor^® Violet 450-Goat Anti-Rabbit IgG(H+L)	500 μL , 120 μL , 1 mL
E-AB-1194	HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed	500 μL , 120 μL , 1 mL
E-IR-R217	2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution)	50 mL , 18 mL , 6 mL , 3 mL
E-IR-R220	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0)	10mL , 3mL
E-IR-R221	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0)	10mL , 3mL
E-IR-R304A	Western Blot Detection Kit	50 Assays
E-IR-R304B	High Accuracy and Absorbability Western Blot Detection Kit	50 Assays