HA-Tag Polyclonal Antibody-Elabscience伊莱瑞特【官网】

HA-Tag Polyclonal Antibody

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货号：E-AB-40523
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说明书

说明书

Price: ¥780.00 ¥300.00

Size:

100 μL 25 μL

宿主： Rabbit
反应性： All
应用性： WB;IF;IP;FCM

Verified Samples	Verified Samples in WB: lane1: HA tag transfected HEK 293 whole cell lysate, lane2: HEK 293whole cell lysate Verified Samples in IF: 293F Verified Samples in IP: 293F Verified Samples in FCM: 293F
Dilution	WB 1:2000-5000, IP 3μg/sample, IF 1:800-1600, FCM 0.2μg
Clonality	Polyclonal
Isotype	IgG
Immunogen	Synthetic peptide corresponding to HA tag conjugated to keyhole limpet haemocyanin.
Abbre	HA-Tag
Synonyms	HA1, HA2, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, HA epitope tag
Calculated MW	28 kDa
Observed MW	28 kDa
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS, pH 7.2. Contains 0.05% proclin 300.
Purification Method	Antigen Affinity Purification
Conjugation	Unconjugated
Background	The HA tag is derived from the HA-molecule corresponding to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to int

Western blotting with Anti-HA rabbit polyclonal antibody at dilution of 1:1000. Lane1: HA tag transfected HEK 293 whole cell lysate, Lane2: HEK 293 whole cell lysate

Immunofluorescent analysis of 293F cells transfected with the HA-GFP, using anti-HA-Tag Polyclonal Antibody at 1:800 dilution.

IP Result of 293F cells transfected with HA-Tag fusion protein,using anti-HA-Tag rabbit polyclonal antibody. lane 1: rabbit IgG Isotype Control, Lane 2:input, Lane 3: anti-HA-Tag rabbit antibody

1x10^6 CHO cells Transfected with a HA plasmid were stained with 0.2ug Anti-HA tag pAb(E-AB-40523) and AF647 conjugated Goat Anti-Rabbit IgG (H+L);lsotype Control stained with 0.2ug Rabbit IgG and AF647 conjugated Goat Anti-Rabbit IgG (H+L)

Microglial Pdcd4 deficiency mitigates neuroinflammation-associated depression via facilitating Daxx mediated PPARγ/IL-10 signaling

IF: 9.300

Journal： Journal of Neuroinflammation (2024)

DOI：10.1186/s12974-024-03142-3

Reactivity：Mouse

Sample Type：brain tissue

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E-AB-1010	Goat Anti-Rabbit IgG(H+L)(Cyanine3 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1014	Goat Anti-Rabbit IgG(H+L)(FITC conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1043	Streptavidin(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
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E-AB-1075	Goat Anti-Rabbit IgG(H+L)(Elab Fluor® 647 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1099	Elab Fluor® Violet 450-Goat Anti-Rabbit IgG(H+L)	500 μL , 120 μL , 1 mL
E-AB-1194	HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed	500 μL , 120 μL , 1 mL
E-IR-R217	2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution)	50 mL , 18 mL , 6 mL , 3 mL
E-IR-R220	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0)	10mL , 3mL
E-IR-R221	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0)	10mL , 3mL
E-IR-R304A	Western Blot Detection Kit	50 Assays
E-IR-R304B	High Accuracy and Absorbability Western Blot Detection Kit	50 Assays