HMGB1 Polyclonal Antibody
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Price:
Size:
200 μL
120 μL
60 μL
- 宿主： Rabbit
- 反应性： Human;Mouse;Rat
- 应用性： WB;IHC;IF
| Verified Samples |
Verified Samples in WB: Human nasopharyngeal, SW480, B16, Human Nasopharyngeal, Human liver cancer, Human colon cancer, Mouse skeletal muscle, Mouse kidney, Rat kidney Verified Samples in IHC: Human liver cancer, Mouse kidney, Human tonsil Verified Samples in IF: Mouse heart |
| Dilution | WB 1:500-1:2000, IHC 1:200-1:800, IF 1:200-1:800 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse HMGB1 |
| Abbre | HMGB1 |
| Synonyms | Amphoterin, Chromosomal protein, DKFZp686A04236, HMG-1, HMG1, HMG3, HMGB 1, HMGB1, High mobility group 1, High mobility group box 1, High mobility group protein 1, High mobility group protein B1, high-mobility group (nonhistone chromosomal) protein 1, nonhistone |
| Swissprot | |
| Calculated MW | 25 kDa |
| Observed MW | 25 kDa |
| Cellular Localization | Nucleus. Chromosome. Cytoplasm. Secreted. Cell membrane. Endosome. Endoplasmic reticulum-Golgi intermediate compartment. In basal state predominantly nuclear. Shuttles between the cytoplasm and the nucleus (PubMed:12231511, PubMed:17114460). Translocates from the nucleus to the cytoplasm upon autophagy stimulation (PubMed:20819940). Release from macrophages in the extracellular milieu requires the activation of NLRC4 or NLRP3 inflammasomes (By similarity). Passively released to the extracellular milieu from necrotic cells by diffusion, involving the fully reduced HGMB1 which subsequently gets oxidized (PubMed:19811284). Also released from apoptic cells (PubMed:16855214, PubMed:18631454). Active secretion from a variety of immune and non-immune cells such as macrophages, monocytes, neutrophils, dendritic cells and natural killer cells in response to various stimuli such as LPS and cytokines involves a nonconventional secretory process via secretory lysosomes (PubMed:12231511, PubMed:14532127, PubMed:15944249). Secreted by plasma cells in response to LPS (By similarity). Found on the surface of activated platelets (PubMed:11154118). |
| Concentration | 1.45 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.02% sodium azide, 1% BSA and 50% glycerol, pH 7.4. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Epigenetics and Nuclear Signaling |
| Conjugation | Unconjugated |
| Background | High mobility group (HMG) proteins 1 and 2 are ubiquitous non-histone components of chromatin.Evidence suggests that the binding of HMG proteins to DNA induces alterations in the DNA architecture including DNA bending and unwinding of the helix.HMG proteins synergize with Oct-2, members of the NF B family, ATF-2 and c-Jun to activate transcription.Other studies indicate that phosphorylation of HMG protein is required to stimulate the transcriptional activity of the protein.Human HMG-1 and HMG-2 both contain two DNA-binding domains, termed HMG boxes.HMG proteins bind single-stranded DNA but induce conformational changes in double-stranded DNA alone. |
Western Blot analysis of various samples using HMGB1 Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry analysis of paraffin-embedded Human liver cancer using HMGB1 Polyclonal Antibody at dilution of 1:500.
Immunohistochemistry analysis of paraffin-embedded mouse kidney using HMGB1 Polyclonal Antibody at dilution of 1:500.
Immunofluorescence analysis of paraffin-embedded Mouse heart using HMGB1 Polyclonal Antibody at dilution of 1:300.
Immunohistochemistry analysis of paraffin-embedded Human Tonsil using HMGB1 Polyclonal Antibody(Elabscience Product Detected by Lifespan).
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