(KO Validated) STAT3 Polyclonal Antibody
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- 宿主： Rabbit
- 反应性： Human;Mouse;Rat
- 应用性： WB;IF
| Verified Samples |
Verified Samples in WB:HeLa, 293T Verified Samples in IF: Rat brain, Mouse brain |
| Dilution | WB 1:500-1:2000, IF 1:50-1:200 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein of human STAT3 (NP_644805.1). |
| Abbre | STAT3 |
| Synonyms | ADMIO, ADMIO1, APRF, HIES, STAT3, Stat3 |
| Swissprot | |
| Calculated MW | 83 kDa/87 kDa/88 kDa |
| Observed MW | 90 kDa |
| Cellular Localization | Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3. Identified in a complex with LYN and PAG1. |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Cardiovascular, Epigenetics and Nuclear Signaling, Developmental Biology, Signal Transduction, Stem Cells |
| Conjugation | Unconjugated |
| Background | The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
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