(KO Validated) Vimentin Polyclonal Antibody-Elabscience伊莱瑞特【官网】

(KO Validated) Vimentin Polyclonal Antibody

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货号：E-AB-63601
文献（4）
说明书

说明书

(KO Validated) Vimentin Polyclonal Antibody

Price: ¥4020.00 ¥2520.00 ¥1580.00

Size:

200 μL 120 μL 60 μL

宿主： Rabbit
反应性： Human;Mouse;Rat
应用性： WB;IHC;IF

Verified Samples	Verified Samples in WB: Jurkat, 293T, HeLa, Mouse lung, Mouse testis, Rat lung Verified Samples in IHC: Human tonsil, Human stomach Verified Samples in IF: L929
Dilution	WB 1:500-1:2000, IHC 1:50-1:200, IF 1:50-1:200
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant fusion protein of human Vimentin (NP_003371.2).
Abbre	Vimentin
Synonyms	CTRCT30, HEL113, VIM, Vimentin, vimentin
Swissprot	P08670
Calculated MW	53 kDa
Observed MW	57 kDa
Cellular Localization	Cytoplasm
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Purification Method	Affinity purification
Research Areas	Cancer, Developmental biology, Neuroscience, Signaling transduction, Stem cells
Conjugation	Unconjugated
Background	This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.

Western blot analysis of extracts of various cell lines using Vimentin Polyclonal Antibody at dilution of 1:1000.

Western blot analysis of extracts from normal (control) and Vimentin knockout (KO) 293T cells using Vimentin Polyclonal Antibody at dilution of 1:1000.

Immunohistochemistry of paraffin-embedded Human tonsil using Vimentin Polyclonal Antibody at dilution of 1:150 (40x lens).

Immunohistochemistry of paraffin-embedded Human stomach using Vimentin Polyclonal Antibody at dilution of 1:150 (40x lens).

Immunofluorescence analysis of L929 cells using Vimentin Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

A multifunctional silk-hyaluronic acid self-healing hydrogel laden with alternatively activated macrophage-derived exosomes reshape microenvironment of diabetic wound and accelerate healing

IF: 7.700

Journal： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024)

DOI：10.1016/j.ijbiomac.2024.132384

Reactivity：Rat
Expression of Markers Associated with Epithelial—Mesenchymal Transition and Extracellular Matrix Degradation in Human Uveal Melanoma

IF: 0.900

Journal： BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE (2024)

DOI：10.1007/s10517-024-06266-x

Reactivity：Human

Sample Type：choroid tissue without tumor
Quinacrine inhibits cMET-mediated metastasis and angiogenesis in breast cancer stem cells

IF: 4.100

Journal： Journal of Cell Communication and Signaling (2023)

DOI：10.1007/s12079-023-00756-9

PMID：37162635

Reactivity：Human
Hypermethylation of the GRHL2 promoter region is associated with ovarian endometriosis

IF: 3.923

Journal： REPRODUCTION (2022)

DOI：10.1530/REP-21-0383

Reactivity：Human

E-AB-1003	Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1010	Goat Anti-Rabbit IgG(H+L)(Cyanine3 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1014	Goat Anti-Rabbit IgG(H+L)(FITC conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1043	Streptavidin(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1060	Goat Anti-Rabbit IgG(H+L)(Elab Fluor® 594 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1075	Goat Anti-Rabbit IgG(H+L)(Elab Fluor® 647 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1099	Elab Fluor® Violet 450-Goat Anti-Rabbit IgG(H+L)	500 μL , 120 μL , 1 mL
E-AB-1194	HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed	500 μL , 120 μL , 1 mL
E-IR-R217	2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution)	50 mL , 18 mL , 6 mL , 3 mL
E-IR-R220	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0)	10mL , 3mL
E-IR-R221	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0)	10mL , 3mL
E-IR-R304A	Western Blot Detection Kit	50 Assays
E-IR-R304B	High Accuracy and Absorbability Western Blot Detection Kit	50 Assays