MDM2 Polyclonal Antibody-Elabscience伊莱瑞特【官网】

MDM2 Polyclonal Antibody

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货号：E-AB-31995
文献（3）
说明书

说明书

Price: ¥2365.00 ¥1640.00 ¥1010.00 ¥420.00

Size:

200 μL 120 μL 60 μL 20 μL

宿主： Rabbit
反应性： Human;Mouse;Monkey
应用性： WB;IHC-p;IF

Verified Samples	Verified Samples in WB: Hela
Dilution	WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000
Clonality	Polyclonal
Isotype	IgG
Immunogen	Synthesized peptide derived from human MDM2 around the non-phosphorylation site of Ser166.
Abbre	MDM2
Synonyms	ACTFS, Double minute 2 protein, E3 ubiquitin-protein ligase Mdm2, HDMX, Hdm 2, Hdm2, MDM 2, MDM2, MDM2 oncogene E3 ubiquitin protein ligase, Mdm2 p53 E3 ubiquitin protein ligase homolog, Mdm2 transformed 3T3 cell double minute 2 p53 binding protein (mouse) binding
Swissprot	Q00987
Calculated MW	55 kDa
Observed MW	90 kDa
Cellular Localization	Nucleus>nucleoplasm. Cytoplasm. Nucleus>nucleolus. Expressed predominantly in the nucleoplasm. Interaction with ARF(P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF(P14) and MDM2 may be necessary to allow efficient nucleolar localization of both proteins. Colocalizes with RASSF1 isoform A in the nucleus.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH 7.4.
Purification Method	Affinity purification
Research Areas	Cancer, Cell Biology, Epigenetics and Nuclear Signaling
Conjugation	Unconjugated
Background	This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells.

Western Blot analysis of Hela cells using MDM2 Polyclonal Antibody at dilution of 1:2000.

MDM2 inhibitor APG-115 synergizes with ABT-199 to induce cell apoptosis in chronic lymphocytic leukemia

IF: 4.400

Journal： Frontiers in Pharmacology (2024)

DOI：10.3389/fphar.2024.1441383

Reactivity：Human
Dapagliflozin alleviates arsenic trioxide-induced hepatic injury in rats via modulating PI3K/AkT/mTOR, STAT3/SOCS3/p53/MDM2 signaling pathways and miRNA-21, miRNA-122 expression

IF: 5.600

Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2023)

DOI：10.1016/j.intimp.2023.111325

PMID：38070468

Reactivity：Rat

Sample Type：liver tissue
Functional role and epithelial to mesenchymal transition of the miR-590-3p/MDM2 axis in hepatocellular carcinoma

IF: 3.800

Journal： BMC CANCER (2023)

DOI：10.1186/s12885-023-10861-y

PMID：37138218

Reactivity：Human

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E-AB-1010	Goat Anti-Rabbit IgG(H+L)(Cyanine3 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1014	Goat Anti-Rabbit IgG(H+L)(FITC conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1043	Streptavidin(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
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E-AB-1075	Goat Anti-Rabbit IgG(H+L)(Elab Fluor® 647 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1099	Elab Fluor® Violet 450-Goat Anti-Rabbit IgG(H+L)	500 μL , 120 μL , 1 mL
E-AB-1194	HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed	500 μL , 120 μL , 1 mL
E-IR-R217	2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution)	50 mL , 18 mL , 6 mL , 3 mL
E-IR-R220	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0)	10mL , 3mL
E-IR-R221	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0)	10mL , 3mL
E-IR-R304A	Western Blot Detection Kit	50 Assays
E-IR-R304B	High Accuracy and Absorbability Western Blot Detection Kit	50 Assays