METTL3 Polyclonal Antibody-Elabscience伊莱瑞特【官网】

METTL3 Polyclonal Antibody

说明书

Price: ¥3580.00 ¥2220.00 ¥1380.00

Size:

200 μL 120 μL 60 μL

Verified Samples	Verified Samples in WB: HepG2, Mouse spleen, Mouse thymus Verified Samples in IF: C6, HeLa, NIH/3T3, Hela, U-2OS
Dilution	WB 1:500-1:2000, IF 1:50-1:200
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant fusion protein of human METTL3 (NP_062826.2).
Abbre	METTL3
Synonyms	IME4, M6A, METTL3, MT-A70, Spo8, hMETTL3
Swissprot	Q86U44
Calculated MW	25 kDa/64 kDa
Observed MW	80 kDa
Cellular Localization	Nucleus speckle. Colocalizes with speckles in interphase nuclei. Suggesting that it may be associated with nuclear pre-mRNA splicing components.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Purification Method	Affinity purification
Research Areas	Cancer, Epigenetics and Nuclear Signaling
Conjugation	Unconjugated
Background	This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine.

Western blot analysis of extracts of various cell lines using METTL3 Polyclonal Antibody at dilution of 1:1000.

Immunofluorescence analysis of C6 cells using METTL3 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using METTL3 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using METTL3 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Confocal immunofluorescence analysis of Hela cells using METTL3 Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.

Confocal immunofluorescence analysis of U-2 OS cells using METTL3 Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.

E-AB-1003	Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1010	Goat Anti-Rabbit IgG(H+L)(Cyanine3 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1014	Goat Anti-Rabbit IgG(H+L)(FITC conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1043	Streptavidin(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1060	Goat Anti-Rabbit IgG(H+L)(Elab Fluor® 594 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1075	Goat Anti-Rabbit IgG(H+L)(Elab Fluor® 647 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1099	Elab Fluor® Violet 450-Goat Anti-Rabbit IgG(H+L)	500 μL , 120 μL , 1 mL
E-AB-1194	HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed	500 μL , 120 μL , 1 mL
E-IR-R217	2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution)	50 mL , 18 mL , 6 mL , 3 mL
E-IR-R220	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0)	10mL , 3mL
E-IR-R221	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0)	10mL , 3mL
E-IR-R304A	Western Blot Detection Kit	50 Assays
E-IR-R304B	High Accuracy and Absorbability Western Blot Detection Kit	50 Assays