Myc-Tag Monoclonal Antibody

Price:
Size:
100 μL
25 μL
- 宿主: Rabbit
- 反应性: All
- 应用性: WB;IF;IP
Verified Samples |
Verified Samples in WB: MYC-Tag fused recombinant protein, 293F Verified Samples in IP: 293F |
Dilution | WB 1:2000-5000, IP 3ug/sample, IF 1:400-800 |
Clonality | Monoclonal |
Isotype | IgG |
Immunogen | Synthetic peptide corresponding to Myc tag conjugated to keyhole limpet haemocyanin. |
Abbre | Myc-Tag |
Synonyms | Myc Epitope Tag, Myc proto-oncogene protein, Transcription factor p64, c Myc Tag, c Myc, c Myc Epitope Tag |
Calculated MW | 28 kDa |
Observed MW | 28 kDa |
Concentration | 1 mg/mL |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
Purification Method | Protein A/G Purification |
Clone No. | 2G8 |
Conjugation | Unconjugated |
Background | The MYC tag, derived from the c-MYC protein, is a popular epitope tag for detecting the expression of recombinant proteins in yeast, bacteria, insect, and mammalian cell systems. It can be used for affinity chromatography, then used to separate recombin |
Western blotting with anti-MYC rabbit monoclonal antibody at dilution of 1:5000.Lane 1: MYC tag transfected HEK 293 cell lysates, lane 2: HEK 293 whole cell lysate
Immunofluorescent analysis of 293F cells transfected with Myc-Tag fusion protein, using anti-Myc-Tag rabbit monoclonal antibody at dilution of 1:200.
IP Result of 293F cells transfected with Myc-Tag fusion protein,using anti-Myc-Tag rabbit monoclonal antibody . Lane 1:input, lane 2: rabbit IgG Isotype Control, Lane 3: anti-Myc-Tag rabbit monoclonal antibody