One-step TUNEL Flow Cytometry Apoptosis Kit (Green, FITC)

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中文名称：一步法TUNEL流式凋亡检测试剂盒（绿色，FITC）

货号：E-CK-A420
文献（9）
说明书

说明书

One-step TUNEL Flow Cytometry Apoptosis Kit (Green, FITC)

Price: ¥3600.00 ¥2000.00 ¥1500.00

Size:

100Assays 50Assays 20Assays

应用： 细胞凋亡-DNA片段化

检测原理	细胞在发生凋亡时，会激活一些特异性的DNA内切酶，这些内切酶会切断核小体间的基因组DNA。使得凋亡细胞的DNA被切割成180~200bp片段，在琼脂糖凝胶上通常以180~200bp的阶梯状迁移。TdT酶（脱氧核糖核酸末端转移酶）将标记的dUTP连接到断裂DNA暴露的3'-OH末端，通过在这些末端添加荧光dUTP的方式来标记晚期凋亡细胞，从而可以通过流式细胞仪进行检测。
检测方法	荧光法;流式
样本类型	细胞
检测时间	2.5-3 hours
检测仪器	流式细胞仪
荧光	FITC
Ex/Em	490/530
Channel set	FITC
自备试剂	PBS(含1%BSA)缓冲液(pH7.2-7.4),PBS（含1% Saponin）缓冲液（pH7.2-7.6）,4%多聚甲醛（溶剂为PBS，pH7.2-7.6）(E-IR-R113),双蒸水
保存温度	该产品可在-20°C避光条件下保存12个月。
有效期	12个月
运输条件	冰袋

Camptothecin-induced apoptosis in HL-60 cells. Test: HL-60 cells were treated with 2.5 μM Camptothecin for 4h. Control: Normal HL-60 cells were not treated with drugs. Negative: HL-60 cells were treated with 2.5 μM camptothecin for 4h without TdT enzyme.

Flow cytometry analysis of camptothecin-induced apoptosis in Jarket cells.

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