One-step TUNEL Flow Cytometry Apoptosis Kit (Green, FITC)

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中文名称：一步法TUNEL流式凋亡检测试剂盒（绿色，FITC）

货号：E-CK-A420
文献（10）
说明书

说明书

One-step TUNEL Flow Cytometry Apoptosis Kit (Green, FITC)

Price: ¥3600.00 ¥2000.00 ¥1500.00

Size:

100 Assays 50 Assays 20 Assays

应用： 细胞凋亡-DNA片段化

检测原理	细胞在发生凋亡时，会激活一些特异性的DNA内切酶，这些内切酶会切断核小体间的基因组DNA。使得凋亡细胞的DNA被切割成180~200bp片段，在琼脂糖凝胶上通常以180~200bp的阶梯状迁移。TdT酶（脱氧核糖核酸末端转移酶）将标记的dUTP连接到断裂DNA暴露的3'-OH末端，通过在这些末端添加荧光dUTP的方式来标记晚期凋亡细胞，从而可以通过流式细胞仪进行检测。
检测方法	荧光法;流式
样本类型	细胞
检测时间	2.5-3 hours
检测仪器	流式细胞仪
荧光	FITC
Ex/Em	490/530
Channel set	FITC
自备试剂	PBS(含1%BSA)缓冲液(pH7.2-7.4),PBS（含1% Saponin）缓冲液（pH7.2-7.6）,4%多聚甲醛（溶剂为PBS，pH7.2-7.6）(E-IR-R113),双蒸水
保存温度	该产品可在-20°C避光条件下保存12个月。
有效期	12个月
运输条件	冰袋

Camptothecin-induced apoptosis in HL-60 cells. Test: HL-60 cells were treated with 2.5 μM Camptothecin for 4h. Control: Normal HL-60 cells were not treated with drugs. Negative: HL-60 cells were treated with 2.5 μM camptothecin for 4h without TdT enzyme.

Flow cytometry analysis of camptothecin-induced apoptosis in Jarket cells.

Proteomic analysis reveals QiShenYiQi Pills ameliorates ischemia-induced heart failure through inhibition of mitochondrial fission

IF: 6.7

Journal： PHYTOMEDICINE (2025)

DOI：10.1016/j.phymed.2025.156435
A smart-responsive Y-shaped DNA scaffold drug conjugate achieving precise and synergetic tumor chemo-gene therapy via promoting tumor apoptosis and activating the anti-tumor immunity

IF: 13.3

Journal： CHEMICAL ENGINEERING JOURNAL (2024)

DOI：10.1016/j.cej.2024.157036
Legumain deficiency halts atherogenesis by modulating T cell receptor signaling

IF: 8.0

Journal： AGING CELL (2024)

DOI：10.1111/acel.14391
Network pharmacology and transcriptomic profiling elucidate the therapeutic effects of Ranunculus ternatus Thunb on liver fibrosis via MK3-NF-κB inhibition

IF: 5.2

Journal： Aging-US (2024)

DOI：10.18632/aging.205629

PMID：38461449
Protective role of forsythoside B in Kawasaki disease-induced cardiac injury: Inhibition of pyroptosis via the SIRT1–NF-κB–p65 signaling pathway

IF: 5.1

Journal： CHEMICO-BIOLOGICAL INTERACTIONS (2024)

DOI：10.1016/j.cbi.2024.110953

PMID：38471628
The LCHADD Mouse Model Recapitulates Early-Stage Chorioretinopathy in LCHADD Patients

IF: 5.0

Journal： INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE (2024)

DOI：10.1167/iovs.65.6.33
Putrescine supplementation improves the developmental competence of in vitro produced bovine embryos

IF: 2.4

Journal： THERIOGENOLOGY (2024)

DOI：10.1016/j.theriogenology.2024.10.017
Glutathione carbon dots as an intracellular reactive oxygen species scavenger for reducing cisplatin-induced ototoxicity

IF: 8.6

Journal： VIEW (2023)

DOI：10.1002/VIW.20230056
Small EV in plasma of triple negative breast cancer patients induce intrinsic apoptosis in activated T cells

IF: 5.9

Journal： Communications Biology (2023)

DOI：10.1038/s42003-023-05169-3

PMID：37542121
The protective effect of lncRNA NEAT1/miR-122-5p/Wnt1 axis on hippocampal damage in hepatic ischemic reperfusion young mice

IF: 4.800

Journal： CELLULAR SIGNALLING (2023)

DOI：10.1016/j.cellsig.2023.110668

PMID：37004832

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