Phospho-AMPK alpha1/2 (Thr183/172) Polyclonal Antibody
Price:
- 宿主： Rabbit
- 反应性： Human;Mouse;Rat;Monkey
- 应用性： WB;IHC-p;IF
| Verified Samples |
Verified Samples in WB: L929, Rat kidney, Mouse brain Verified Samples in IHC: Mouse brain Verified Samples in IF: Rat heart |
| Dilution | WB 1:500-1:2000, IHC 1:100-1:300, IF 1:50-1:200 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthesized peptide derived from human AMPKα1/2 around the phosphorylation site of Thr183/172 |
| Abbre | AMPKα1/2 (phospho Thr183/172) |
| Synonyms | 5'-AMP-activated protein kinase catalytic subunit alpha-1, ACACA kinase, AMPK, AMPK subunit alpha-1, AMPK1, Acetyl-CoA carboxylase kinase, HMGCR kinase, Hydroxymethylglutaryl-CoA reductase kinase, PRKAA1, PRKAA2, Tau-protein kinase PRKAA1 |
| Swissprot | |
| Calculated MW | 62 kDa |
| Observed MW | 63 kDa |
| Cellular Localization | Cytosol, Nucleus, nuclear speck, nucleoplasm, nucleus, Plasma Membrane, apical plasma membrane, Other locations: cytoplasm, intracellular, nucleotide-activated protein kinase complex. |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH 7.4. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Cardiovascular, Metabolism, Neuroscience, Signal Transduction |
| Conjugation | Unconjugated |
| Background | AMPK (for 5'-AMP-activated protein kinase) is a heterotrimeric complex comprising a catalytic α subunit and regulatory β and γ subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation.Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. |
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Inhibition of lactate dehydrogenase A by diclofenac sodium induces apoptosis in HeLa cells through activation of AMPK
IF: 5.500 -
Cytarabine prevents neuronal damage by enhancing AMPK to stimulate PINK1 / Parkin-involved mitophagy in Parkinson's disease model
IF: 4.200 -
Krüppel-like factor 10 modulates stem cell phenotypes of pancreatic adenocarcinoma by transcriptionally regulating notch receptors
IF: 11.000 -
SIRT1 activation promotes energy homeostasis and reprograms liver cancer metabolism
IF: 7.400 -
Resveratrol downregulates ENaCs through the activation of AMPK in human colon cancer cells
IF: 2.600 -
Sex Differences in Neuropathy: The Paradigmatic Case of MetFormin
IF: 6.208Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)PMID：36498830Reactivity：MouseSample Type：sciatic nerves -
Therapeutic Effects of Omentin-1 on Pulmonary Fibrosis by Attenuating Fibroblast Activation via AMP-Activated Protein Kinase Pathway
IF: 4.757
