RAF1 Polyclonal Antibody
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- 宿主： Rabbit
- 反应性： Human;Mouse;Rat
- 应用性： WB;IHC;IF
| Verified Samples |
Verified Samples in WB: 293T, Jurkat, HeLa, MCF-7, HepG2, Mouse liver Verified Samples in IHC: Rat liver, Rat heart Verified Samples in IF: U2OS |
| Dilution | WB 1:500-1:2000, IHC 1:50-1:200, IF 1:50-1:200 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein of human RAF1 (NP_002871.1). |
| Abbre | RAF1 |
| Synonyms | CMD1NN, CRAF, NS5, RAF1, Raf-1, c-Raf |
| Swissprot | |
| Calculated MW | 73 kDa/75 kDa |
| Observed MW | 73 kDa |
| Cellular Localization | Cytoplasm. Cell membrane. Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes. |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Cell Biology, Metabolism, Signal Transduction |
| Conjugation | Unconjugated |
| Background | This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. |
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The antipsychotic drug pimozide promotes apoptosis through the RAF/ERK pathway and enhances autophagy in breast cancer cells
IF: 3.600Journal： CANCER BIOLOGY & THERAPY (2024)PMID：38356266Reactivity：HumanSample Type：breast cancer tissue -
IL-35 enhances angiogenic effects of small extracellular vesicles in breast cancer
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