α-SMA Polyclonal Antibody（E-AB-34268）|-Elabscience伊莱瑞特【官网】

α-SMA Polyclonal Antibody

一键复制产品信息

货号：E-AB-34268
文献（35）
说明书

说明书

Price: ¥2365.00 ¥1640.00 ¥1010.00 ¥420.00

Size:

200 μL 120 μL 60 μL 20 μL

宿主： Rabbit
反应性： Human;Mouse;Rat
应用性： WB;IHC-p;IF

Verified Samples	Verified Samples in WB: 3T3, Hela Verified Samples in IHC: Rat lung Verified Samples in IF: Mouse heart
Dilution	WB 1:500-2000, IHC 1:50-300, IF 1:50-1:200
Clonality	Polyclonal
Isotype	IgG
Immunogen	Synthesized peptide derived from α-SMA
Abbre	α-SMA
Synonyms	AAT6, ACTA, ACTA2, ACTSA, ACTVS, Actin, Actin alpha 2 smooth muscle aorta, Actin aortic smooth muscle, Alpha 2 actin, Alpha actin 2, Alpha cardiac actin, Alpha-actin-2, Cell growth inhibiting gene 46 protein, Cell growth-inhibiti, a actin, alpha sma, aortic smooth muscle
Swissprot	P62736, P68133, P63267
Calculated MW	42 kDa
Observed MW	42 kDa
Cellular Localization	Cytoskeleton, actin filament, cytoskeleton, filamentous actin, Cytosol, Extracellular region or secreted, blood microparticle, extracellular exosome, extracellular matrix, extracellular space, Nucleus, Plasma Membrane, Other locations: dense body, focal adhesion, membrane, myelin sheath, myofibril, phagocytic vesicle.
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH 7.4.
Purification Method	Affinity purification
Research Areas	Cancer, Cardiovascular, Developmental Biology, Signal Transduction, Stem Cells
Conjugation	Unconjugated
Background	Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 (Actin, Alpha 2, Smooth Muscle, Aorta) is a Protein Coding gene. Diseases associated with ACTA2 include Multisystemic Smooth Muscle Dysfunction Syndrome and Moyamoya Disease 5. Among its related pathways are ICos-ICosL Pathway in T-Helper Cell and GPCR Pathway. GO annotations related to this gene include protein kinase binding. An important paralog of this gene is ACTG2. ACTA1 (Actin, Alpha 1, Skeletal Muscle) is a Protein Coding gene. Diseases associated with ACTA1 include Nemaline Myopathy 3, Autosomal Dominant Or Recessive and Myopathy, Congenital, With Fiber-Type Disproportion. Among its related pathways are ICos-ICosL Pathway in T-Helper Cell and GPCR Pathway. GO annotations related to this gene include structural constituent of cytoskeleton and myosin binding. An important paralog of this gene is ACTC1. ACTG2 (Actin, Gamma 2, Smooth Muscle, Enteric) is a Protein Coding gene. Diseases associated with ACTG2 include Visceral Myopathy and Chronic Intestinal Pseudoobstruction. Among its related pathways are ICos-ICosL Pathway in T-Helper Cell and GPCR Pathway. An important paralog of this gene is ACTA2.

Western Blot analysis of 3T3, Hela cells using α-SMA Polyclonal Antibody at dilution of 1:1500.

Immunohistochemistry of paraffin-embedded Rat lung tissue using α-SMA Polyclonal Antibody at dilution of 1:200.

Immunofluorescence analysis of Mouse heart tissue using α-SMA Polyclonal Antibody at dilution of 1:200.

Therapeutic use of fisetin and pirfenidone combination in bleomycin-induced pulmonary fibrosis in adult male albino rats

IF: 3.1

Journal： NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2024)

DOI：10.1007/s00210-024-03363-6

Reactivity：Rat

Sample Type：Lung tissue
Distribution and morphologic characterization of telocytes in rat ovary and uterus: insights from ultrastructural and immunohistochemical analysis

IF: 2.1

Journal： HISTOCHEMISTRY AND CELL BIOLOGY (2024)

DOI：10.1007/s00418-024-02313-w

Reactivity：Rat

Sample Type：ovarian stroma,endometrial stroma
Celastrol-based nanoporous membranes prevent subconjunctival fibrosis by activating autophagy

IF: 10.000

Journal： Materials Today Advances (2023)

DOI：10.1016/j.mtadv.2023.100356

Reactivity：Human

Sample Type：pterygium tissue
A new approach for the treatment of bleomycin-induced rat pulmonary injury by combined protein fraction of major royal jelly protein 2 and its isoform X1

IF: 7.5

Journal： BIOMEDICINE & PHARMACOTHERAPY (2023)

DOI：10.1016/j.biopha.2023.115578

PMID：37742609

Reactivity：Rat

Sample Type：lung tissue
Osteomatrix as a personalized 3D tissue-specific invasion test-bed for oral carcinoma

IF: 6.600

Journal： Biomaterials Science (2023)

DOI：10.1039/D2BM01870E

PMID：37057646

Reactivity：Human
Modulatory effect of rupatadine on mesenchymal stem cell-derived exosomes in hepatic fibrosis in rats: A potential role for miR-200a

IF: 6.100

Journal： LIFE SCIENCES (2023)

DOI：10.1016/j.lfs.2023.121710

PMID：37084952

Reactivity：Rat

Sample Type：liver
A novel mechanistic approach for the anti-fibrotic potential of rupatadine in rat liver via amendment of PAF/NF-?B p65/TGF-β1 and hedgehog/HIF-1α/VEGF trajectories

IF: 5.800

Journal： INFLAMMOPHARMACOLOGY (2023)

DOI：10.1007/s10787-023-01147-7

PMID：36811777

Reactivity：Rat

Sample Type：liver
Human Colonoid–Myofibroblast Coculture for Study of Apical Na+/H+ Exchangers of the Lower Cryptal Neck Region

IF: 5.600

Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023)

DOI：10.3390/ijms24054266

PMID：36901695

Reactivity：Human

Sample Type：colonoids
Liver fibrosis is a common pathological change in the liver of dairy cows with fatty liver

IF: 3.500

Journal： JOURNAL OF DAIRY SCIENCE (2023)

DOI：10.3168/jds.2022-22021

PMID：36823013

Reactivity：Bovine

Sample Type：liver
Npc1 gene mutation abnormally activates the classical Wnt signalling pathway in mouse kidneys and promotes renal fibrosis

IF: 2.4

Journal： ANIMAL GENETICS (2023)

DOI：10.1111/age.13381

PMID：38087834

Reactivity：Mouse

Sample Type：kidney tissue

E-AB-1003	Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1043	Streptavidin(peroxidase/HRP conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1010	Goat Anti-Rabbit IgG(H+L)(Cyanine3 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1014	Goat Anti-Rabbit IgG(H+L)(FITC conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1060	Goat Anti-Rabbit IgG(H+L)(Elab Fluor® 594 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-IR-R304A	Western Blot Detection Kit	50 Assays
E-IR-R304B	High Accuracy and Absorbability Western Blot Detection Kit	50 Assays
E-IR-R217	2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution)	50 mL , 18 mL , 6 mL , 3 mL
E-IR-R220	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0)	10 mL , 3 mL
E-IR-R221	Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0)	10 mL , 3 mL
E-AB-1075	Goat Anti-Rabbit IgG(H+L)(Elab Fluor® 647 conjugated)	500 μL , 120 μL , 60 μL , 30 μL , 1 mL
E-AB-1194	HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed	500 μL , 120 μL , 1 mL
E-AB-1099	Elab Fluor® Violet 450-Goat Anti-Rabbit IgG(H+L)	500 μL , 120 μL , 1 mL