TUNEL In Situ Apoptosis Kit (HRP-DAB Method)

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中文名称：TUNEL原位细胞凋亡检测试剂盒（HRP-DAB显色法）

货号：E-CK-A331
文献（122）
说明书

说明书

TUNEL In Situ Apoptosis Kit (HRP-DAB Method)

Price: ¥4620.00 ¥2850.00 ¥1650.00

Size:

100 Assays 50 Assays 20 Assays

应用： 细胞凋亡-DNA片段化

检测原理	细胞在发生凋亡时，会激活一些DNA内切酶，剪断核小体间的基因组DNA，暴露的3’-OH可在末端脱氧核酸转移酶（TdT）的催化下加上生物素标记的dUTP，辣根过氧化物酶(HRP)标记的Streptavidin (Streptavidin-HRP)可与之结合，在HRP的催化下通过DAB显色可观测到凋亡细胞，从而可通过普通光学显微镜检测到细胞的凋亡。由于正常的或正在增殖的细胞中几乎没有DNA的断裂，因而没有3'-OH形成，很少能够被标记。这就是TUNEL(TdT-mediated dUTP Nick-End Labeling)法检测细胞凋亡的原理。
检测方法	比色法
样本类型	石蜡切片;冰冻切片
检测时间	3 hours
检测仪器	普通光学显微镜
自备试剂	4%多聚甲醛(E-IR-R113),0.2% Triton X-100(E-IR-R122),3% H2O2(E-IR-R115),二甲苯，乙醇，DNase I，PBS(E-BC-R187),ddH2O,苏木素(E-IR-R120)，中性树胶(E-IR-R118)
保存温度	该产品可在-20°C避光条件下保存12个月。
有效期	12个月
运输条件	冰袋

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