TUNEL In Situ Apoptosis Kit (HRP-DAB Method)
中文名称：TUNEL原位细胞凋亡检测试剂盒（HRP-DAB显色法）
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- 应用： 细胞凋亡-DNA片段化
| 检测原理 | 细胞在发生凋亡时，会激活一些DNA内切酶，剪断核小体间的基因组DNA，暴露的3’-OH可在末端脱氧核酸转移酶（TdT）的催化下加上生物素标记的dUTP，辣根过氧化物酶(HRP)标记的Streptavidin (Streptavidin-HRP)可与之结合，在HRP的催化下通过DAB显色可观测到凋亡细胞，从而可通过普通光学显微镜检测到细胞的凋亡。由于正常的或正在增殖的细胞中几乎没有DNA的断裂，因而没有3'-OH形成，很少能够被标记。这就是TUNEL(TdT-mediated dUTP Nick-End Labeling)法检测细胞凋亡的原理。 |
| 检测方法 | 比色法 |
| 样本类型 | 石蜡切片;冰冻切片 |
| 检测时间 | 3 hours |
| 检测仪器 | 普通光学显微镜 |
| 自备试剂 | 4%多聚甲醛(E-IR-R113),0.2% Triton X-100(E-IR-R122),3% H2O2(E-IR-R115),二甲苯，乙醇，DNase I，PBS(E-BC-R187),ddH2O,苏木素(E-IR-R120)，中性树胶(E-IR-R118) |
| 保存温度 | 该产品可在-20°C避光条件下保存12个月。 |
| 有效期 | 12个月 |
| 运输条件 | 冰袋 |
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| E-CK-A211 | Annexin V-FITC/PI Apoptosis Kit | 200 Assays , 100 Assays , 50 Assays , 20 Assays |
| E-CK-A301 | Mitochondrial Membrane Potential Assay Kit (with JC-1) | 100 Assays , 50 Assays , 20 Assays |
| E-CK-A362 | Enhanced Cell Counting Kit 8 (WST-8/CCK8) | 500 Tests , 100 Tests , 500 Tests × 20 |
| E-CK-A320 | One-step TUNEL In Situ Apoptosis Kit (Green, FITC) | 100 Assays , 50 Assays , 20 Assays |
