Annexin V-APC/7-AAD Apoptosis Kit

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中文名称：Annexin V-APC / 7-AAD细胞凋亡检测试剂盒

货号：E-CK-A218
文献（81）
说明书

说明书

Price: ¥3100.00 ¥2100.00 ¥1350.00 ¥850.00

Size:

200 Assays 100 Assays 50 Assays 20 Assays

应用： 细胞凋亡-Annexin V

检测原理	Annexin V是一种钙离子依赖性磷脂结合蛋白，与磷脂酰丝氨酸PS有高度亲和力，可通过细胞外侧暴露的PS与凋亡早期细胞胞膜结合，将Annexin V标记荧光染料，利用流式细胞仪检测细胞凋亡。细胞核染料（PI,DAPI,7-AAD）可与双链DNA特异性结合，并产生强烈的荧光，正常情况下无法透过细胞膜。由于凋亡晚期或坏死细胞膜丧失完整性，细胞核染料（DAPI、PI、7-AAD）可进入细胞内对DNA进行染色，与Annexin V搭配使用，可区分处于不同凋亡时期的细胞。
检测方法	荧光法;流式
样本类型	细胞
检测时间	40 min
检测仪器	流式细胞仪
荧光	APC
Ex/Em	650/660
Channel set	APC
自备试剂	PBS(E-BC-R187), 去离子水
保存温度	该产品可在 2~8°C避光条件下保存12个月。
有效期	12个月
运输条件	冰袋

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E-CK-A217	Annexin V-APC/PI Apoptosis Kit	200 Assays , 100 Assays , 50 Assays , 20 Assays
E-CK-A151	Annexin V Binding Buffer (10×)	50 mL , 10 mL
E-CK-A301	Mitochondrial Membrane Potential Assay Kit (with JC-1)	100 Assays , 50 Assays , 20 Assays
E-CK-A258	Annexin V-APC/DAPI Apoptosis Kit	200 Assays , 100 Assays , 50 Assays , 20 Assays
E-CK-A320	One-step TUNEL In Situ Apoptosis Kit (Green, FITC)	100 Assays , 50 Assays , 20 Assays