Annexin V-APC/7-AAD Apoptosis Kit

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中文名称：Annexin V-APC / 7-AAD细胞凋亡检测试剂盒

货号：E-CK-A218
文献（81）
说明书

说明书

Price: ¥3100.00 ¥2100.00 ¥1350.00 ¥850.00

Size:

200 Assays 100 Assays 50 Assays 20 Assays

应用： 细胞凋亡-Annexin V

检测原理	Annexin V是一种钙离子依赖性磷脂结合蛋白，与磷脂酰丝氨酸PS有高度亲和力，可通过细胞外侧暴露的PS与凋亡早期细胞胞膜结合，将Annexin V标记荧光染料，利用流式细胞仪检测细胞凋亡。细胞核染料（PI,DAPI,7-AAD）可与双链DNA特异性结合，并产生强烈的荧光，正常情况下无法透过细胞膜。由于凋亡晚期或坏死细胞膜丧失完整性，细胞核染料（DAPI、PI、7-AAD）可进入细胞内对DNA进行染色，与Annexin V搭配使用，可区分处于不同凋亡时期的细胞。
检测方法	荧光法;流式
样本类型	细胞
检测时间	40 min
检测仪器	流式细胞仪
荧光	APC
Ex/Em	650/660
Channel set	APC
自备试剂	PBS(E-BC-R187), 去离子水
保存温度	该产品可在 2~8°C避光条件下保存12个月。
有效期	12个月
运输条件	冰袋

Exploring the immunological mechanism of Houttuynia cordata in the treatment of colorectal cancer through combined network pharmacology and experimental validation

IF: 3.1

Journal： NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2024)

DOI：10.1007/s00210-024-03203-7
The immunotherapy mechanism of Hedyotis Diffusae Herba in treating liver cancer: a study based on network pharmacology, bioinformatics, and experimental validation

IF: 3.1

Journal： NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2024)

DOI：10.1007/s00210-024-03312-3
Pericyte loss via glutaredoxin2 downregulation aggravates diabetes-induced microvascular dysfunction

IF: 3.0

Journal： EXPERIMENTAL EYE RESEARCH (2024)

DOI：10.1016/j.exer.2024.110025
SIRT1 silencing promotes EMT and Crizotinib resistance by regulating autophagy through AMPK/mTOR/S6K signaling pathway in EML4-ALK L1196M and EML4-ALK G1202R mutant non-small cell lung cancer cells

IF: 3.0

Journal： MOLECULAR CARCINOGENESIS (2024)

DOI：10.1002/mc.23799
The m6A methyltransferase METTL5 promotes neutrophil extracellular trap network release to regulate hepatocellular carcinoma progression

IF: 2.9

Journal： Cancer Medicine (2024)

DOI：10.1002/cam4.7165
DNA hypermethylation of COL4A1 in ultraviolet-B-induced age-related cataract models in vitro and in vivo

IF: 1.9

Journal： International Journal of Ophthalmology (2024)

DOI：10.18240/ijo.2024.10.04
Warburg effect enhanced by AKR1B10 promotes acquired resistance to pemetrexed in lung cancer-derived brain metastasis

IF: 7.4

Journal： Journal of Translational Medicine (2023)

DOI：10.1186/s12967-023-04403-0

PMID：37587486
Overexpression of PRDX2 in Adipose-Derived Mesenchymal Stem Cells Enhances the Therapeutic Effect in a Neurogenic Erectile Dysfunction Rat Model by Inhibiting Ferroptosis

IF: 7.310

Journal： Oxidative Medicine and Cellular Longevity (2023)

DOI：10.1155/2023/4952857

PMID：36819780

Reactivity：Rat
Mechanism of Bazhen decoction in the treatment of colorectal cancer based on network pharmacology, molecular docking, and experimental validation

IF: 7.3

Journal： Frontiers in Immunology (2023)

DOI：10.3389/fimmu.2023.1235575

PMID：37799727
Targeting the up-regulated CNOT3 reverses therapeutic resistance and metastatic progression of EGFR-mutant non-small cell lung cancer

IF: 7.0

Journal： Cell Death Discovery (2023)

DOI：10.1038/s41420-023-01701-w

PMID：37919290

E-CK-A117	Annexin V-APC Reagent	500 Tests , 200 Tests , 100 Tests , 50 Tests
E-CK-A217	Annexin V-APC/PI Apoptosis Kit	200 Assays , 100 Assays , 50 Assays , 20 Assays
E-CK-A151	Annexin V Binding Buffer (10×)	50 mL , 10 mL
E-CK-A301	Mitochondrial Membrane Potential Assay Kit (with JC-1)	100 Assays , 50 Assays , 20 Assays
E-CK-A258	Annexin V-APC/DAPI Apoptosis Kit	200 Assays , 100 Assays , 50 Assays , 20 Assays
E-CK-A320	One-step TUNEL In Situ Apoptosis Kit (Green, FITC)	100 Assays , 50 Assays , 20 Assays